Coding

Part:BBa_K1197014:Design

Designed by: Alişan Kayabölen   Group: iGEM13_METU_Turkey   (2013-10-02)


House fly cytochrome P450 reductase (fCPR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1769


Design Notes

It is used as natural. Biobrick prefix and suffix were added by primers.


Source

Its source is Musca domestica genome.

References

Andersen, J.F., Utermohlen, J.G., Feyereisen, R., 1994. Expression of house fly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system. Biochemistry 33, 2171e2177.